DSMZ 171 . THERMOANAEROBACTER KIVUI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.22 g 0.216 1.241
KH2PO4 0.22 g 0.216 1.588
NaH2PO4 x H2O 4.50 g 4.42 36.844
Na2HPO4 x 12 H2O 6.10 g 5.992 42.21
NH4Cl 0.31 g 0.305 5.693
(NH4)2SO4 0.22 g 0.216 1.635
NaCl 0.45 g 0.442 7.564
MgSO4 x 7 H2O 0.09 g 0.088 0.359
CaCl2 x 2 H2O
(0.1% w/v)
6.00 ml 0.006 0.04
FeSO4 x 7 H2O solution (0.1% w/v) 2.00 ml - -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
L-Cysteine HCl x H2O 0.50 g 0.491 2.796
Na2S x 9 H2O 0.50 g 0.491 2.045
Distilled water 1000.00 ml - -
1 Dissolve ingredients except cysteine and sulfide, sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.5.
2 After inoculation pressurize cultivation vessels to one bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 7 other media (first defined in Medium 119).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1 g 1 3.597
H2SO4
(0.1 N)
1000 ml - -
1 The ferrous sulfate solution is not stable and should be freshly prepared.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 5.992 42.21
NaH2PO4 x H2O 4.42 36.844
L-Cysteine HCl x H2O 0.491 2.796
Na2S x 9 H2O 0.491 2.045
NH4Cl 0.305 5.693
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain