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DSMZ

DSMZ 1717 . PERLABENTIMONAS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Tenuifilum thalassicum DSM 100343

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 50 °C

Main sol. 1717 #

ml
Equipment needed: Filter Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.330 g 0.33 2.425
NH4Cl 0.330 g 0.33 6.169
KCl 0.330 g 0.33 4.427
NaCl 10.000 g 10 171.116
MgCl2 x 6 H2O 0.330 g 0.33 1.623
CaCl2 x 6 H2O 0.033 g 0.033 0.151
NaHCO3 2.000 g 2 23.808
Yeast extract 0.050 g 0.05 -
Tryptone 2.000 g 2 28.137
Fructose 2.000 g 2 11.101
Resazurin 1.000 mg 1.0e-3 0.004
Modified Wolin's mineral solution 10.000 ml - -
Wolin's vitamin solution 10.000 ml - -
Na2S x 9 H2O 0.500 g 0.5 2.082
Distilled water 1000.000 ml - -
1 Dissolve ingredients (except carbonate, and sulfide), adjust pH to 8.0, boil for 10 min. and sparge medium with 100% N2 gas for 30 min to make it anoxic. Distribute under N2 into Hungate tubes (10 ml) and autoclave. Add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube. Adjust pH of the complete medium to 8.0, if necessary.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 02.05.22
Source: DSMZ
Synonyms: 1717.PERLABENTIMONAS MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Tenuifilum thalassicum DSM 100343

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 2 28.137
NaHCO3 2 23.808
Fructose 2 11.101
Na2S x 9 H2O 0.5 2.082
NH4Cl 0.33 6.169
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Cultivation metadata from 2 strains BacDive

halophily halophilic 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 2 strains
nutrition chemoorganoheterotroph 1 strain
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