1717 .
PERLABENTIMONAS MEDIUM
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Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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KH2PO4 | 0.330 | g | ||
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NH4Cl | 0.330 | g | ||
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KCl | 0.330 | g | ||
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NaCl | 10.000 | g | ||
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MgCl2 x 6 H2O | 0.330 | g | ||
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CaCl2 x 6 H2O | 0.033 | g | ||
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NaHCO3 | 2.000 | g | ||
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Yeast extract | 0.050 | g | ||
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Tryptone | 2.000 | g | ||
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Fructose | 2.000 | g | ||
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Resazurin | 1.000 | mg | ||
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Modified Wolin's mineral solution | 10.000 | ml | ||
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Wolin's vitamin solution | 10.000 | ml | ||
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Na2S x 9 H2O | 0.500 | g | ||
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Distilled water | 1000.000 | ml | ||
| 1 Dissolve ingredients (except carbonate, and sulfide), adjust pH to 8.0, boil for 10 min. and sparge medium with 100% N2 gas for 30 min to make it anoxic. Distribute under N2 into Hungate tubes (10 ml) and autoclave. Add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube. Adjust pH of the complete medium to 8.0, if necessary. | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
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Biotin | 2.0 | mg |
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium D-(+)-pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
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(DL)-alpha-Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |
Modified Wolin's mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Nitrilotriacetic acid | 1.50 | g | ||
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MgSO4 x 7 H2O | 3.00 | g | ||
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MnSO4 x H2O | 0.50 | g | ||
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NaCl | 1.00 | g | ||
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FeSO4 x 7 H2O | 0.10 | g | ||
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CoSO4 x 7 H2O | 0.18 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
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ZnSO4 x 7 H2O | 0.18 | g | ||
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CuSO4 x 5 H2O | 0.01 | g | ||
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AlK(SO4)2 x 12 H2O | 0.02 | g | ||
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H3BO3 | 0.01 | g | ||
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Na2MoO4 x 2 H2O | 0.01 | g | ||
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2SeO3 x 5 H2O | 0.30 | mg | ||
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Na2WO4 x 2 H2O | 0.40 | mg | ||
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Distilled water | 1000.00 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||