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DSMZ

DSMZ 1719 . R2A WITH ARTIFICIAL SODA-SALT LAKE WATER

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1719 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 46.94 g 46.94 803.217
Na2SO4 7.84 g 7.84 55.196
MgCl2 x 6 H2O 0.24 g 0.24 1.181
CaCl2 x 2 H2O 0.17 g 0.17 1.156
KCl 1.38 g 1.38 18.511
KBr 0.19 g 0.19 1.597
H3BO3 0.05 g 0.05 0.809
SrCl2 x 6 H2O 0.08 g 0.08 0.3
NaF 0.06 g 0.06 1.429
Distilled water 900.00 ml - -
1 Add 3 g / L R2A (See medium 830).
2 Autoclave to sterilize.
3 Add aseptically 100 mL sterile Na2Co3 solution (50 g / L, autoclaved).
4 pH 9.0.

Medium 830 #

1000
Solution occurs in 9 other media (first defined in Medium 830).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g - -
Proteose peptone
(Difco no. 3) 		0.50 g - -
Casamino acids 0.50 g - -
Glucose 0.50 g - -
Starch
(soluble) 		0.50 g - -
Na-pyruvate 0.30 g - -
K2HPO4 0.30 g - -
MgSO4 x 7 H2O 0.05 g - -
Agar 15.00 g - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Last modified: 02.04.22
Source: DSMZ
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 46.94 803.217
Na2SO4 7.84 55.196
KCl 1.38 18.511
MgCl2 x 6 H2O 0.24 1.181
KBr 0.19 1.597
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain