1720
1720 .
Mineral medium (Jagmann)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1720 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 1.000 | g | 1 | 13.414 | |
| NaCl | 0.400 | g | 0.4 | 6.845 | |
| NH4Cl | 0.270 | g | 0.27 | 5.048 | |
| MgSO4 x 7 H2O | 0.060 | g | 0.06 | 0.243 | |
| CaCl2 | 1.000 | mg | 1.0e-3 | 0.009 | |
| K2HPO4 | 0.018 | g | 0.018 | 0.103 | |
| NaH2PO4 | 6.000 | mg | 0.006 | 0.05 | |
|
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) |
4.700 | g | 4.7 | 19.723 | |
| Trace element solution SL-10 | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Dissolve and autoclave. pH should be 7.0-7.2. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |