1721
1721 .
MMJHS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1721 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaNO3 | 1 | g | 1 | 11.765 | |
| NaHCO3 | 1 | g | 1 | 11.904 | |
| Na2S2O3 x 5 H2O | 1 | g | 1 | 4.029 | |
|
Sulfur
(powdered) |
10 | g | 10 | 311.818 | |
|
Synthetic seawater
(MJ) |
1000 | ml | - | - | |
| 1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3. | |||||
| 2 Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa. The final pH is 7.0. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 25.00 | g | 25 | 427.789 |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 |
| CaCl2 x 2 H2O | 0.70 | g | 0.7 | 4.761 |
| NH4Cl | 0.25 | g | 0.25 | 4.674 |
| MgSO4 x 7 H2O | 3.40 | g | 3.4 | 13.794 |
| MgCl2 x 6 H2O | 4.20 | g | 4.2 | 20.659 |
| KCl | 0.50 | g | 0.5 | 6.707 |
| Trace mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.500 | g | 0.5 | - | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 2.000 | mg | 0.002 | 0.008 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2WO4 x 2 H2O | 0.100 | g | 0.1 | 0.303 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
| Distilled water | 1000.000 | ml | - | - | |
| Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
| Na2SeO3 x 5 H2O | 0.050 | g | 0.05 | 0.19 | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| MgCl2 x 6 H2O | 4.2 | 20.659 |
| NaHCO3 | 1 | 11.904 |
| NaNO3 | 1 | 11.765 |
| Na2S2O3 x 5 H2O | 1 | 4.029 |
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