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DSMZ

DSMZ 1729 . MEDIUM FOR THIORHODOVIBRIO FRISIUS

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1729 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.25 g 0.25 1.837
NH4Cl 0.34 g 0.34 6.356
KCl 0.34 g 0.34 4.561
MgSO4 x 7 H2O 0.50 g 0.5 2.029
CaCl2 x 2 H2O 0.25 g 0.25 1.701
NaCl 20.00 g 20 342.231
MgCl2 x 6 H2O 2.80 g 2.8 13.773
Sodium acetate 0.25 g 0.25 3.048
Trace element solution SL-12 B 1.00 ml - -
Double distilled water 1000.00 ml - -
1 Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas.
2 Autoclave at 121 °C for 15 min.
3 aftre autoclaving add the following sterile solutions
3% NaHCO3
(autoclaved) 		50.00 ml/l - -
10-Vitamins-solution (1000x)
(filter sterilized) 		1.00 ml/l - -
Vitamin B12 solution 0.02 g/L
(filter sterilized) 		1.00 ml/l - -
Na2S x 9 H2O
(1M in water, autoclaved) 		1.25 ml/l 0.3 1.25
4 Adjust pH to 7.2.
5 Add 1 ml/100ml of culture Sulfide feeding solution (Siefert and Pfennig 1984) every 3 days or when the sulfide is consumed.

3% NaHCO3 #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1.5 g 30 357.113
Double distilled water 50.0 ml - -
1 Fill 50 ml in 100 ml serum bottles, bubble for 15 min with CO2 and autoclave bottle capped tightly at 121°C for 15 min

Trace element solution SL-12 B #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 8.923
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 2 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -
1 Adjust pH to 6.0.
2 Filtrate (filter sterilze).

10-Vitamins-solution (1000x) #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
DL-Calcium pantothenate 50 mg 0.05 0.105
Vitamin B12
(Cyanocobalamin) 		1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -
1 Filtrate (filter sterilze).

Vitamin B12 solution 0.02 g/L #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12
(Cyanocobalamin) 		2 mg 0.02 0.015
Distilled water 100 ml - -
1 Filtrate (filter sterilze).

Sulfide feeding solution (Siefert and Pfennig 1984) #

250
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 7.00 g 28 116.578
Na2CO3 2.65 g 10.6 100.011
Distilled water 250.00 ml - -
1 Sparge medium with 100% CO2 gas mixture.
2 Autoclave at 121 °C for 15 min.
3 Sterile solution is saturated with CO2 (0.8 bar) until pH is approx. 7.3.
4 1 ml is added to 100 ml of culture approx. every 3 days or when the sulfide is consumed.

Metadata

Last modified: 15.05.23
Source: DSMZ
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
MgCl2 x 6 H2O 2.8 13.773
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.5 2.029
NH4Cl 0.34 6.356
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