Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.25 | g | 0.25 | 1.837 | |
NH4Cl | 0.34 | g | 0.34 | 6.356 | |
KCl | 0.34 | g | 0.34 | 4.561 | |
MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
NaCl | 20.00 | g | 20 | 342.231 | |
MgCl2 x 6 H2O | 2.80 | g | 2.8 | 13.773 | |
Sodium acetate | 0.25 | g | 0.25 | 3.048 | |
Trace element solution SL-12 B | 1.00 | ml | - | - | |
Double distilled water | 1000.00 | ml | - | - | |
1 Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. | |||||
2 Autoclave at 121 °C for 15 min. | |||||
3 aftre autoclaving add the following sterile solutions | |||||
3% NaHCO3
(autoclaved) |
50.00 | ml/l | - | - | |
10-Vitamins-solution (1000x)
(filter sterilized) |
1.00 | ml/l | - | - | |
Vitamin B12 solution 0.02 g/L
(filter sterilized) |
1.00 | ml/l | - | - | |
Na2S x 9 H2O
(1M in water, autoclaved) |
1.25 | ml/l | 0.3 | 1.25 | |
4 Adjust pH to 7.2. | |||||
5 Add 1 ml/100ml of culture Sulfide feeding solution (Siefert and Pfennig 1984) every 3 days or when the sulfide is consumed. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaHCO3 | 1.5 | g | 30 | 357.113 | |
Double distilled water | 50.0 | ml | - | - | |
1 Fill 50 ml in 100 ml serum bottles, bubble for 15 min with CO2 and autoclave bottle capped tightly at 121°C for 15 min |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 3.0 | g | 3 | 8.923 | |
FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
MnCl2 x 2 H2O | 50.0 | mg | 0.05 | - | |
ZnCl2 | 42.0 | mg | 0.042 | 0.308 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 18.0 | mg | 0.018 | 0.074 | |
H3BO3 | 300.0 | mg | 0.3 | 4.851 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 6.0. | |||||
2 Filtrate (filter sterilze). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 | |
Folic acid | 20 | mg | 0.02 | 0.045 | |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 | |
Thiamine HCl | 50 | mg | 0.05 | 0.148 | |
Riboflavin | 50 | mg | 0.05 | 0.133 | |
Nicotinic acid | 50 | mg | 0.05 | 0.406 | |
DL-Calcium pantothenate | 50 | mg | 0.05 | 0.105 | |
Vitamin B12
(Cyanocobalamin) |
1 | mg | 1.0e-3 | 7.4e-4 | |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 | |
Lipoic acid | 50 | mg | 0.05 | 0.242 | |
Distilled water | 1000 | ml | - | - | |
1 Filtrate (filter sterilze). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12
(Cyanocobalamin) |
2 | mg | 0.02 | 0.015 | |
Distilled water | 100 | ml | - | - | |
1 Filtrate (filter sterilze). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2S x 9 H2O | 7.00 | g | 28 | 116.578 | |
Na2CO3 | 2.65 | g | 10.6 | 100.011 | |
Distilled water | 250.00 | ml | - | - | |
1 Sparge medium with 100% CO2 gas mixture. | |||||
2 Autoclave at 121 °C for 15 min. | |||||
3 Sterile solution is saturated with CO2 (0.8 bar) until pH is approx. 7.3. | |||||
4 1 ml is added to 100 ml of culture approx. every 3 days or when the sulfide is consumed. |
Last modified: | 15.05.23 |
Source: | DSMZ |
Medium type: | Defined medium |
Final pH: | 6.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 3 |