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DSMZ

DSMZ 1733 . MEDIUM FOR HYDROGENOPHILUS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1733 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.3 g 2.599 19.097
Na2HPO4 x 6 H2O 2.9 g 3.277 23.083
NH4Cl 1.0 g 1.13 21.124
NaHCO3 0.5 g 0.565 6.725
CaCl2 x 2 H2O 0.1 g 0.113 0.769
Trace element solution SL 6 5.0 ml - -
Distilled water 880.0 ml - -
1 Mix components thoroughly, adjust pH to 6.8. Distribute the medium into culture vessels (e.g., 17.0 ml in 120 ml serum bottles), seal with butyl rubber stoppers and autoclave.
2 After cooling, add the following autoclaved solutions (for final 20 ml):
MgSO4 x 7 H2O
(0.5%) 		2.0 ml 0.01 0.041
Fe(NH4)citrate
(0.1%) 		0.4 ml 4.0e-4 -
Sodium lactate
(10%) 		0.4 ml 0.04 0.357
Yeast extract
(1%) 		0.2 ml 0.002 -
3 Pressurize to 100 kPa with H2-CO2 (4:1, v/v).
4 Add 10% O2 to headspace

Trace element solution SL 6 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 6 H2O 3.277 23.083
KH2PO4 2.599 19.097
NH4Cl 1.13 21.124
NaHCO3 0.565 6.725
CaCl2 x 2 H2O 0.113 0.769
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain