1749
1749 .
Sucrose-Asparagine Medium
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1749 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Mineral Salts Solution with Asparagin (L) | 990 | ml | - | - |
| Sucrose Solution (10 mL) | 10 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.5 | g | 0.505 | 2.049 | |
| K2HPO4 | 1.0 | g | 1.01 | 5.799 | |
| L-Asparagin | 2.0 | g | 2.02 | 15.291 | |
| Tap water | 990.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15.152 | 45.05 | |
| 1 Adjust to pH 7.0 sterilize by autoclaving. The medium may be solidified by adding 15.0 g/l agar. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sucrose | 20 | g | 2000 | 5842.83 | |
| 1 Sterilize by autoclaving. | |||||
| 2 After autoclaving add 10 mL of the Sucrose solution to the Mineral Salts Solution with Asparagine (final volume 1 L). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 7 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sucrose | 20 | 58.428 |
| Agar | 15 | 44.599 |
| L-Asparagin | 2 | 15.138 |
| K2HPO4 | 1 | 5.741 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| Show more | ||
Cultivation metadata from 7 strains
| phyla | 2 | 10 strains |
| oxygen | obligate aerobe | 4 strains |
| oxygen | aerobe | 3 strains |
| temperature | mesophilic | 4 strains |