174 .
ZUELZERA MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
D-Glucose | 5.0 | g | ||
|
|
Yeast extract
(OXOID) |
2.0 | g | ||
|
|
Peptone
(BD BACTO) |
2.0 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
Na-thioglycolate | 0.5 | g | ||
|
|
Agar
, for stabs
optional |
10.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients, adjust pH to 7.6 with KOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
| 2 For stabs add 10.0 g/l agar. | |||||