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DSMZ

DSMZ 1778a . ANB aerobic

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1778a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 15 g/l 15 141.525
NaHCO3 20 g/l 20 238.075
NaCl 3 g/l 3 51.335
K2HPO4 1 g/l 1 5.741
Trace element solution 1 ml - -
1 mix compounds.
2 check pH (should be 10)
3 Fill 100 mL each into 200 mL serum-flasks.
4 Autoclave at 121 °C for 20 min.
5 After autoclaving, add the following sterile solutions:
MgSO4
(1M in H2O) 		1 ml/l 0.12 1
NH4Cl
(2M in H2O) 		2 ml/l 0.214 4
Vitamine mixture 1 ml/l - -
Melezitose
(10% in H2O) 		10 ml/l 2 -
Yeast extract
(1% solution in water) 		2 ml/l 0.02 -
6 Mix well and aliquot into the desired volumes and flasks or tubes (e.g., 20 ml in 100 ml flasks).

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

Vitamine mixture #

400
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acidic thiamine / Ca pantothenate solution 100 ml - -
Basic vitamin solution 100 ml - -
Neutral folic acid / riboflavin solution 100 ml - -
Neutral B12 solution 0.005 g/L 100 ml - -
1 Sterilize by filtration.
2 Mix the four solutions in the ratio 1:1:1:1.

Acidic thiamine / Ca pantothenate solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 5 mg 0.05 0.188
Calcium pantothenate 5 mg 0.05 0.105
Distilled water
(acidified to pH 3.0 with HCl) 		100 ml - -

Basic vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA
(p-aminobenzoic acid) 		5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
NaOH
(0.1N) 		100 ml - -

Neutral folic acid / riboflavin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Neutral B12 solution 0.005 g/L #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

Metadata

Last modified: 02.08.24
Source: MediaDive: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
Description:

used for aerobic moderately haloalkalilphilic Planctomycetota strain AB-hyl4 (DSM 117794)
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 20 238.075
Na2CO3 15 141.525
NaCl 3 51.335
Melezitose 2 -
K2HPO4 1 5.741
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