Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
Casamino acids
(BD BBL) |
1.00 | g | 1 | - | |
KH2PO4 | 3.10 | g | 3.1 | 22.78 | |
(NH4)2SO4 | 2.50 | g | 2.5 | 18.919 | |
MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
Allen's trace element solution | 10.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve all compounds except yeast extract and Casamino acids, adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature and autoclave. Prior to inoculation add yeast extract and Casamino acids from stock solutions sterilized by filtration. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MnCl2 x 4 H2O | 180 | mg | 0.18 | 0.91 | |
Na2B4O7 x 10 H2O | 450 | mg | 0.45 | - | |
ZnSO4 x 7 H2O | 22 | mg | 0.022 | 0.077 | |
CuCl2 x 2 H2O | 5 | mg | 0.005 | 0.029 | |
Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
VOSO4 x 2 H2O | 3 | mg | 0.003 | 0.015 | |
CoSO4 x 7 H2O | 1 | mg | 1.0e-3 | 0.006 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH of final solution to 2 with 1 N HCl. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 4.0 - 4.2 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
KH2PO4 | 3.1 | 22.78 |
(NH4)2SO4 | 2.5 | 18.919 |
Casamino acids | 1 | - |
Yeast extract | 1 | - |
CaCl2 x 2 H2O | 0.25 | 1.701 |
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