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DSMZ

DSMZ 1840 . Halorutilales haloarchaeon medium

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1840 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.00 g 240 4106.78
K2HPO4 2.50 g 2.5 14.353
KCl 5.00 g 5 67.069
NH4Cl 0.20 g 0.2 3.739
Trace element solution 1.00 ml - -
Distilled water 999.00 ml - -
1 Adjust pH to 6.8 with 0.5 M KH2PO4.
2 Dispense into serum bottles (30 ml medium in 100 ml serum bottles, OR 15 ml in 50 serum bottles).
3 add 20% CO2 to gas phase (in serum bottles)
4 Autoclave at 121 °C for 20 min.
5 Before inoculation add from sterile stock solutions per liter:
Vitamine mixture 0.50 ml/l - -
MgSO4 x 7 H2O
(1M in water) 		0.08 ml/l 0.02 0.08
alkaline trace metal solution 0.50 ml/l - -
Na-thiosulfate
(2M in water) 		10.00 ml/l 3.162 20
NaHCO3
(8% in water) 		10.50 ml/L 0.84 9.999

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

Vitamine mixture #

400
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acidic thiamine / Ca pantothenate solution 100 ml - -
Basic vitamin solution 100 ml - -
Neutral folic acid / riboflavin solution 100 ml - -
Neutral B12 solution 0.005 g/L 100 ml - -
1 Sterilize by filtration.
2 Mix the four solutions in the ratio 1:1:1:1.

Acidic thiamine / Ca pantothenate solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 5 mg 0.05 0.188
Calcium pantothenate 5 mg 0.05 0.105
Distilled water
(acidified to pH 3.0 with HCl) 		100 ml - -

Basic vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA
(p-aminobenzoic acid) 		5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
NaOH
(0.1N) 		100 ml - -

Neutral folic acid / riboflavin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Neutral B12 solution 0.005 g/L #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

alkaline trace metal solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.040 g/l 0.04 1
Na2SeO3 x 5 H2O 0.026 g/l 0.026 0.099
Na2WO4 x 2 H2O 0.030 g/l 0.03 0.091
Distilled water 1000.000 ml - -

Metadata

Last modified: 02.08.24
Source: MediaDive: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
Description:

used for cultation of lithotrophic thiosulfate-oxidizing Halorutilales haloarchaeon strain col2-1 (DSM 117793)
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 240 4106.78
KCl 5 67.069
Na-thiosulfate 3.162 20
K2HPO4 2.5 14.353
NaHCO3 0.84 9.999
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