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194a. DESULFOTOMACULUM OX39 MEDIUM (XYLENE)

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 930 ml - -
2 Solution B 1 ml - -
3 Solution C 30 ml - -
4 Solution D 20 ml - -
5 Solution E 1 ml - -
6 Solution F 10 ml - -
7 Solution G 10 ml - -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic serum vials (e.g., 50 ml medium in 100 ml serum bottles) and autoclaved. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere.
  2. Solutions E and F are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 7.2 - 7.4.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.40 g 1.502 10.575
2 0.20 g 0.215 1.577
3 0.30 g 0.322 6.018
4 1.00 g 1.073 18.359
5 0.40 g 0.429 2.111
6 0.50 g 0.536 7.196
7 0.15 g 0.161 1.095
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 5.4e-4 0.002
10 930.00 ml 996.065 55289.9
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 ml 12.9 121.509
2 20.0 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.8 g 80 287.754
2
(0.2 N)
10.0 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1
194 DESULFOBULBUS SP. MEDIUM (FRESHWATER)
a DESULFOTOMACULUM OX39 MEDIUM (XYLENE)

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 967.317 53694.1
Na2CO3 1.497 14.124
Na2SO4 1.394 9.815
NaCl 0.996 17.04
FeSO4 x 7 H2O 0.798 2.872
KCl 0.498 6.679
Na2S x 9 H2O 0.399 1.662
MgCl2 x 6 H2O 0.398 1.959
NH4Cl 0.299 5.585
m-Xylene 0.257 2.425
KH2PO4 0.199 1.464
CaCl2 x 2 H2O 0.149 1.016
HCl 0.003 0.087
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Na-resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
CoCl2 x 6 H2O 1.9e-4 8.0e-4
MnCl2 x 4 H2O 1.0e-4 5.0e-4
D-Ca-pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 6.3e-5
p-aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.0e-4
Biotin 2.0e-5 8.2e-5
H3BO3 6.0e-6 9.7e-5
Na-tungstate x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
2,2,4,4,6,8,8-Heptamethylnonane - -
H2SO4 - -

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

Similar media

503. ANAEROBIC FRESHWATER (FWM) MEDIUM 93 %
503b. FWM MEDIUM (MODIFIED) 89 %
728. DESULFOBACCA MEDIUM 88 %
298a. LUPHET1 MEDIUM 88 %
503d. SELENIIVIBRIO MEDIUM (ARSENATE) 87 %

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