Loading ...

195. DESULFOBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 920 ml - -
2 Solution B 1 ml - -
3 Solution C 30 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
6 Solution F 10 ml - -
7 , may stimulate growth of some strains at the beginning
(5% w/v)
20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas mixture into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10%(v/v) inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.254 22.907
2 0.20 g 0.217 1.594
3 0.30 g 0.325 6.083
4 21.00 g 22.777 389.726
5 3.00 g 3.254 16.005
6 0.50 g 0.542 7.274
7 0.15 g 0.163 1.107
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 5.4e-4 0.002
10 920.00 ml 996.042 55288.7
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 1837.15
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 11
Strains with modifications: 11

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.182 55352
NaCl 21.36 365.492
Na2SO4 3.051 21.483
MgCl2 x 6 H2O 3.051 15.01
Na acetate x 3 H2O 2.548 18.727
Show more

Cultivation metadata from 11 strains BacDive

halophily moderate 1 strain
halophily not 1 strain
pH 6 - 8 1 strain
phyla 2 11 strains
oxygen anaerobe 11 strains
Show more

Similar media

195b. DESULFOBULBUS SP. MEDIUM (MARINE) 96 %
195a. DESULFUROMUSA MEDIUM 96 %
383a. DESULFOTIGNUM MEDIUM 96 %
193. DESULFOBACTER MEDIUM 96 %
517. DESULFOBACTERIUM OLEOVORANS MEDIUM 95 %

Latest comments

No comments for this medium.