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195c. DESULFOBACTER SP. MEDIUM (LACTATE)

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Solution A 920.0 ml - -
3 Solution B 1.0 ml - -
4 Solution C 30.0 ml - -
5 Solution D 10.0 ml - -
6 Solution E 10.0 ml - -
7 Solution F 10.0 ml - -
8 , may stimulate growth of some strains
(5% w/v)
20.0 mg/l 0.02 -
9 , might need strain adjustment 1.0 g/l - -
10 Seven vitamins solution , might need strain adjustment 1.0 ml/l - -
11 , might need strain adjustment 0.5 g/l - -
12 , might need strain adjustment 5.0 g/l - -
13 , might need strain adjustment
(DIFCO)
2.0 g/l - -
14 , might need strain adjustment
(BD BBL)
2.0 g/l - -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10%(v/v) inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.254 22.907
2 0.20 g 0.217 1.594
3 0.30 g 0.325 6.083
4 21.00 g 22.777 389.726
5 3.00 g 3.254 16.005
6 0.50 g 0.542 7.274
7 0.15 g 0.163 1.107
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 5.4e-4 0.002
10 920.00 ml 996.042 55288.7
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 2230.95
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 38
Strains with modifications: 38

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.182 55352
NaCl 21.36 365.492
Na2SO4 3.051 21.483
MgCl2 x 6 H2O 3.051 15.01
Na-L-lactate 2.548 22.742
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Cultivation metadata from 38 strains BacDive

halophily not 4 strains
halophily moderate 4 strains
halophily slightly 3 strains
halophily extreme 1 strain
pH 6 - 8 6 strains
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