196 .
DESULFOCAPSA SULFEXIGENS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 961 | ml | ||
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Solution B | 30 | ml | ||
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Solution C | 10 | ml | ||
| 1 First dissolve 2.7 g FeCl3 x 6 H2O in distilled water and adjust the pH to 7.0 with 1 N NaOH (about 3 ml). Add and dissolve remaining salts of solution A and sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 min, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved separately under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be adjusted to 7.2. | |||||
| 2 Note: For transfers use 5 - 10% (v/v) inoculum. | |||||
Solution A #
961
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl3 x 6 H2O | 2.70 | g |
|
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Na2SO4 | 3.00 | g |
|
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KH2PO4 | 0.20 | g |
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NH4Cl | 0.30 | g |
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NaCl | 21.00 | g |
|
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MgCl2 x 6 H2O | 3.00 | g |
|
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KCl | 0.50 | g |
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CaCl2 x 2 H2O | 0.15 | g |
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Trace element solution SL-10 | 1.00 | ml |
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Distilled water | 960.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
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ZnCl2 | 70.0 | mg | ||
|
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MnCl2 x 4 H2O | 100.0 | mg | ||
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H3BO3 | 6.0 | mg | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||