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1a. REACTIVATION WITH LIQUID MEDIUM 1

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 -
2 3 g 3 -
3
optional
15 g 15 -
4 1000 ml 998.207 55408.8
5 , recommended for sporulation of Bacillus strains
optional
10 mg 0.01 0.059
Instructions:
  1. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.
  2. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0

Associated strains

All strains for this medium: 212
Strains with modifications: 14
1 NUTRIENT AGAR
a REACTIVATION WITH LIQUID MEDIUM 1
b GLUTAMINE MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
15 -
Peptone 5 -
Meat extract 3 -
MnSO4 x H2O
optional
0.01 0.059

Cultivation metadata from 212 strains BacDive

halophily slightly 10 strains
halophily not 10 strains
halophily moderate 3 strains
pH 6 - 8 22 strains
pH 8 - 10 19 strains
pH 4 - 6 17 strains
pH 10 - 14 3 strains
phyla 3 211 strains
nutrition chemoorganotroph 1 strain
oxygen aerobe 57 strains
oxygen facultative anaerobe 5 strains
oxygen anaerobe 4 strains
oxygen microaerophile 1 strain
temperature mesophilic 210 strains
temperature psychrophilic 11 strains
temperature thermophilic 7 strains

Similar media

1. NUTRIENT AGAR 100 %
302. NUTRIENT BROTH WITH 10% HORSE SERUM 96 %
238. STREPTOMYCIN NUTRIENT AGAR 96 %
118. DAP-NUTRIENT AGAR 96 %
1b. GLUTAMINE MEDIUM 92 %

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