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|1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.|
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|Agar , for solid medium||15||g||15||44.599|
|2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.|
|Medium type:||Complex medium|
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|Compound||Conc. [g/L]||Conc. [mM]|