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1b. GLUTAMINE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Main sol. 1 1000 ml - -
2 100 mg/l 0.1 0.684
3 100 mg/l 0.1 0.49
Instructions:
  1. To medium 1 add 100 mg/l glutamine and tryptophan (filter-sterilized).

Main sol. 1 #

1000
Solution occurs in 12 other media (first defined in medium 1 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 -
2 3 g 3 -
3
optional
15 g 15 -
4 1000 ml 998.207 55408.8
5 , recommended for sporulation of Bacillus strains
optional
10 mg 0.01 0.059
Instructions:
  1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Metadata

Media status: Official DSMZ medium
Synonyms: Medium 241
Source: DSMZ
Medium type: Complex medium
Final pH: unknown
Equipment needed: Filter

Associated strains

All strains for this medium: No strains found
1 NUTRIENT AGAR
a REACTIVATION WITH LIQUID MEDIUM 1
b GLUTAMINE MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
15 -
Peptone 5 -
Meat extract 3 -
Glutamine 0.1 0.684
Tryptophan 0.1 0.49
MnSO4 x H2O
optional
0.01 0.059

Similar media

1. NUTRIENT AGAR 92 %
1a. REACTIVATION WITH LIQUID MEDIUM 1 92 %
302. NUTRIENT BROTH WITH 10% HORSE SERUM 88 %
238. STREPTOMYCIN NUTRIENT AGAR 88 %
118. DAP-NUTRIENT AGAR 88 %

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