1c
1c .
Nutrient bromothymol blue agar NBTA
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1c #
ml
Equipment needed: Autoclave
sterile filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | - | - | |
| Meat extract | 3 | g | - | - | |
| Agar , for solid medium | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| Bromothymol blue | 25 | mg | - | - | |
| 2,3,5-triphenyltetrazolium chloride stock solution | 4 | ml | - | - | |
| 1 Autoclave at 121 °C for 21 min. | |||||
| 2 After autoclavation add 4 mL from a sterile 2,3,5-triphenyltetrazolium chloride stock solution (1% w/v). | |||||
Metadata
| Last modified: | 27.11.25 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, sterile filter |
| Reference: | doi.org/10.1099/00221287-121-2-303 |
|
Description:
Nutrient agar with bromothymol blue to differtiate phase I and II variants (distinct physiological states in vitro) among Xenorhabdus strains. |
|
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
