205 .
NATRONOBACTERIUM MEDIUM
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Equipment needed:
Final pH:
8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casamino acids | 15.0 | g | ||
|
|
Trisodium citrate x 2 H2O | 3.0 | g | ||
|
|
Glutamic acid | 2.5 | g | ||
|
|
MgSO4 x 7 H2O | 2.5 | g | ||
|
|
KCl | 2.0 | g | ||
|
|
NaCl | 250.0 | g | ||
|
|
Agar | 20.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve agar by heating before adding sodium chloride. Adjust pH to 7.0 before autoclaving. Adjust pH to 8.5 with sterile 5% Na2CO3 after heat sterilization of the medium. | |||||