Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 0.3 | g | ||
|
Na2HPO4 x 12 H2O | 2.1 | g | ||
|
NH4Cl | 1.0 | g | ||
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
Trace element solution | 10.0 | ml | ||
|
FeSO4 x 7 H2O | 1.5 | mg | ||
|
Wolin's vitamin solution | 5.0 | ml | ||
|
Resazurin | 1.0 | mg | ||
|
NaHCO3 | 4.0 | g | ||
|
Yeast extract | 2.0 | g | ||
|
Na-lactate | 5.0 | g | ||
|
Na2S x 9 H2O | 0.5 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | ||
|
FeCl2 x 4 H2O | 0.20 | g | ||
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
CoCl2 x 6 H2O | 0.17 | g | ||
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
ZnCl2 | 0.10 | g | ||
|
CuCl2 | 0.02 | g | ||
|
H3BO3 | 0.01 | g | ||
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
NaCl | 1.00 | g | ||
|
Na2SeO3 x 5 H2O | 0.03 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |