DSMZ 210 . HALOANAEROBIUM MEDIUM

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Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 130.0 g 121.495 2078.98
MgSO4 x 7 H2O 8.8 g 8.224 33.368
KCl 1.0 g 0.935 12.536
Yeast extract 10.0 g 9.346 -
Trypticase
(BBL)
10.0 g 9.346 -
Wolin's vitamin solution 10.0 ml - -
Trace element solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions:
Thioglycolate-ascorbate solution 25.0 ml - -
Glucose
(10 % w/v)
25.0 ml 2.5 13.877
NaOH
(2 N)
10.0 ml - -
2 Final pH: 9.0.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-thioglycolate 0.5 g 20 175.286
Na-ascorbate 0.5 g 20 100.956
Distilled water 25.0 ml - -
1 Filter sterilize.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 8 other media (first defined in Medium 144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Anaerobic workbench, Filter
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 9.346 -
Trypticase 9.346 -
MgSO4 x 7 H2O 8.224 33.368
Glucose 2.5 13.877
KCl 0.935 12.536
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain