210
210 .
HALOANAEROBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 210 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 130.0 | g | 121.495 | 2078.98 | |
| MgSO4 x 7 H2O | 8.8 | g | 8.224 | 33.368 | |
| KCl | 1.0 | g | 0.935 | 12.536 | |
| Yeast extract | 10.0 | g | 9.346 | - | |
|
Trypticase
(BBL) |
10.0 | g | 9.346 | - | |
| Wolin's vitamin solution | 10.0 | ml | - | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions: | |||||
| Thioglycolate-ascorbate solution | 25.0 | ml | - | - | |
|
Glucose
(10 % w/v) |
25.0 | ml | 2.5 | 13.877 | |
|
NaOH
(2 N) |
10.0 | ml | - | - | |
| 2 Final pH: 9.0. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-thioglycolate | 0.5 | g | 20 | 175.286 | |
| Na-ascorbate | 0.5 | g | 20 | 100.956 | |
| Distilled water | 25.0 | ml | - | - | |
| 1 Filter sterilize. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 8
other media (first defined in Medium 144).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.02 | g | 0.02 | 0.149 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Anaerobic workbench, Filter |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 9.346 | - |
| Trypticase | 9.346 | - |
| MgSO4 x 7 H2O | 8.224 | 33.368 |
| Glucose | 2.5 | 13.877 |
| KCl | 0.935 | 12.536 |
| Show more | ||
