Loading ...

213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 950 ml - -
2 Solution B 30 ml - -
3 Solution C 1 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
6 , might need strain adjustment
(2% w/v)
3 g/l - -
Instructions:
  1. Add and dissolve ingredients of solution A, adjust pH to 7.2 and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Solution B is prepared under 80% N2 and 20% CO2 gas atmosphere and autoclaved. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in in the sequence as indicated.
  2. Note: Some cultures are shipped in semi-solid medium which stimulates growth at the beginning. For agar stabs 3.00 g/l agar are added to the complete medium from a sterile anoxic stock solution (2% w/v). Upon receipt add anoxically 1 - 2 ml of the recommended freshly prepared liquid medium to the agar tube and incubate for 3 - 5 days. After incubation transfer 0.5 ml of the resulting cell suspension in the liquid phase to tubes with liquid medium.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(see medium 212)
50.0 ml - -
2 Trace element solution SL-10 1.0 ml - -
3 Clarified rumen fluid 50.0 ml - -
4
(BD BBL)
1.0 g 1.05 -
5 1.7 g 1.786 20.268
6
(0.1% w/v)
0.5 ml 5.3e-4 0.002
7 850.0 ml 891.256 49472.2
8 , might need strain adjustment 1.5 g/l - -
9 , might need strain adjustment 1.7 g/l - -
10 , might need strain adjustment 2.0 g/l - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 170.808
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 32 other media (first defined in medium 1310 )
Equipment needed: Centrifuge Filter Autoclave
Instructions:
  1. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 minand then centrifuged at 27,000 g for 20 min The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at - 20°C.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 6
Strains with modifications: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 897.69 49829.3
Butyric acid 1.695 19.235
Na2CO3 1.499 14.138
Trypticase peptone 0.997 -
L-Cysteine HCl x H2O 0.3 1.706
Show more

Cultivation metadata from 6 strains BacDive

halophily not 2 strains
halophily slightly 1 strain
pH 6 - 8 2 strains
pH 8 - 10 2 strains
pH 4 - 6 1 strain
Show more

Similar media

407. SYNTROPHOMONAS SD2 MEDIUM 82 %
397. FERULATE MEDIUM 74 %
903. CALDANAEROBIUS MEDIUM 71 %
212. SYNTHROPHOMONAS MEDIUM 71 %
336. OXOBACTER PFENNIGII MEDIUM 70 %

Latest comments

No comments for this medium.