DSMZ 220a . REACTIVATION WITH TRYPTONE SOYA BROTH

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Pseudomonas stutzeri DSM 50238

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
220. CASO AGAR (Merck 105458) , alternative 1000 ml - -
545. TRYPTONE SOYA BROTH (TSB) , alternative 1000 ml - -
1 Rehydrate and grow lyophilized cells from the ampoule in liquid tryptone soya broth (see medium 545). Subsequent subculturing may be carried out in liquid medium or on agar plates.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g 15 -
Soy peptone 5 g 5 -
NaCl 5 g 5 85.558
Agar 15 g 15 44.599
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131).
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
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Associated strains

All strains for this medium: 57
Pseudomonas stutzeri DSM 50238

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Casein peptone 17 -
Agar 15 44.599
NaCl 5 85.558
Soy peptone 3 -
K2HPO4 2.5 14.353
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Cultivation metadata from 57 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
pH 4 - 6 1 strain
phyla 2 58 strains
nutrition chemoorganotroph 1 strain
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