221 .
AZOSPIRILLUM MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.05 | g | ||
|
|
K2HPO4 | 0.25 | g | ||
|
|
FeSO4 x 7 H2O | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 1.00 | mg | ||
|
|
MnSO4 x H2O | 2.00 | mg | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
NaCl | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
Biotin | 0.10 | mg | ||
|
|
Agar , if required | 1.50 | % | ||
|
|
Distilled water | 950.00 | ml | ||
|
|
Glucose
(filter-sterilized, 20 %) |
25.00 | ml | ||
|
|
Na malate
(filter-sterilized, 20 %) |
25.00 | ml | ||
| 1 Adjust pH of the medium to 7.1. Add 1.5% agar if needed. Autoclave for 15 min at 121°C. After sterilization add 25 ml each of filter-sterilized 20% glucose and 20% Na-malate. | |||||