228
228 .
PEPTOCOCCUS GLYCINOPHILUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 228 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 5 | - | |
| Peptone | 5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Beef extract | 5.0 | g | 5 | - | |
| Glycine | 3.0 | g | - | - | |
| K2HPO4 | 2.0 | g | 2 | 11.483 | |
| Tween 80 | 1.0 | ml | - | - | |
| Salt solution | 40.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| Haemin solution | 10.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 950.0 | ml | - | - | |
| 1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.1 | ml | - | - | |
|
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 17.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
|
Description:
Medium for Parvimonas micra (synonym Peptococcus glycinophilus) |
|
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .