230
230 .
TRYPTONE YEAST EXTRACT MINERAL MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Slackia heliotrinireducens DSM 20476
This strain uses the standard medium without modifications.
Main sol. 230 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 10.0 | g | 10 | 140.687 | |
| Yeast extract | 10.0 | g | 10 | - | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| MgSO4 | 0.1 | g | 0.1 | 0.831 | |
| CaCl2 | 0.1 | g | 0.1 | 0.901 | |
| NaHCO3 | 6.0 | g | 6 | 71.423 | |
| Resazurin | 0.1 | mg | 1.0e-4 | 4.4e-4 | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.3 | 1.708 | |
| Arginine | 3.0 | g | 3 | 17.222 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
|
Description:
Originally designed for Slackia; Pre-reduced, anaerobic. |
|
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Slackia heliotrinireducens DSM 20476
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 10 | 140.687 |
| Yeast extract | 10 | - |
| NaHCO3 | 6 | 71.423 |
| Arginine | 3 | 17.222 |
| NaCl | 1 | 17.112 |
| Show more | ||
