232 .
MRS MEDIUM WITH CYSTEINE
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* Medium for Lactobacillus acidophilus DSM 20079
Prereduced (oxygen-free); anaerobic
Growth conditions: 37 °C
Equipment needed:
Final pH:
6.2 - 6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone
(tryptic digest) |
10.00 | g | ||
|
|
Meat extract | 10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Glucose | 20.00 | g | ||
|
|
Tween 80 | 1.00 | g | ||
|
|
K2HPO4 | 2.00 | g | ||
|
|
Na-acetate | 5.00 | g | ||
|
|
(NH4)2 citrate | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
MnSO4 x H2O | 0.05 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.2 - 6.5. | |||||
| 2 Add 0.05% cysteine-hydrochloride. | |||||