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DSMZ 232b . WEISSELA KOREENSIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone
(tryptic digest)
10.00 g 10 -
Meat extract 10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 20.00 g 20 111.012
Tween 80 1.00 g 1 0.764
K2HPO4 2.00 g 2 11.483
Na-acetate 5.00 g 5 60.951
(NH4)2 citrate 2.00 g 2 -
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x H2O 0.05 g 0.05 0.296
Distilled water 1000.00 ml - -
1 Adjust pH to 6.2 - 6.5.
2 Add 4 ml Resazurin solution (25 mg/100 ml). 0.05% Cysteine-hydrochloride is added after the medium has been boiled and cooled and CO2. Distribute under N2 and autoclave.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.2 - 6.5
Equipment needed: Autoclave, Gassing station, Hungate tubes

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 20 111.012
Casein peptone 10 -
Meat extract 10 -
Na-acetate 5 60.951
Yeast extract 5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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