234
234 .
EYGA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 234 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1.100 | g | 1.1 | 6.315 | |
| KH2PO4 | 0.860 | g | 0.86 | 6.32 | |
| CaCl2 | 0.025 | g | 0.025 | 0.225 | |
| MgSO4 x 7 H2O | 0.200 | g | 0.2 | 0.811 | |
| NaCl | 0.100 | g | 0.1 | 1.711 | |
| (NH4)2SO4 | 0.500 | g | 0.5 | 3.784 | |
| EDTA/Trace elements mix | 3.000 | ml | - | - | |
| Yeast extract | 1.000 | g | 1 | - | |
| Glucose | 1.000 | g | 1 | 5.551 | |
| Vitamin B12 | 2.000 | µg | 2.0e-6 | 1.5e-6 | |
| Agar | 12.000 | g | 12 | 35.679 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 6.8. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.000 | g | 8.333 | 28.515 |
| ZnSO4 x 7 H2O | 2.200 | g | 3.667 | 12.751 |
| MnSO4 x 4 H2O | 0.570 | g | 0.95 | 4.259 |
| FeSO4 x 7 H2O | 0.500 | g | 0.833 | 2.997 |
| CoCl2 x 6 H2O | 0.161 | g | 0.268 | 2.067 |
| CuSO4 x 5 H2O | 0.157 | g | 0.262 | 1.048 |
| Na2MoO4 x 2 H2O | 0.151 | g | 0.252 | 1.04 |
| Distilled water | 600.000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
|
Description:
Originally designed for Arthrobacter globiformis and other coryneform bacteria |
|
| Edit in Medium builder | |