DSMZ 248 . OAT FLAKES AGAR WITH YEAST EXTRACT

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Streptomyces gramineus DSM 42058

This strain uses the standard medium without modifications.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Oat flakes 20 g 20 -
Trace element solution 1 ml - -
Yeast extract 2 g 2 -
Agar 20 g 20 -
Distilled water 1000 ml - -
1 Adjust pH to 7.2.
2 Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar.
Solution occurs in 8 other media (first defined in Medium 84).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Metadata

Last modified: 19.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 29
Streptomyces gramineus DSM 42058

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 -
Oat flakes 20 -
Yeast extract 2 -
MnCl2 x 4 H2O 1.0e-3 0.005
FeSO4 x 7 H2O 1.0e-3 0.004
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Cultivation metadata from 29 strains BacDive

pH 4 - 6 3 strains
pH 8 - 10 3 strains
phyla 3 30 strains
oxygen aerobe 8 strains
temperature mesophilic 25 strains
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