Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Tryptone
(BD BACTO) |
5.00 | g | 5 | 70.343 | |
Yeast extract | 0.50 | g | 0.5 | - | |
KH2PO4 | 1.36 | g | 1.36 | 9.994 | |
K2HPO4 | 6.95 | g | 6.95 | 39.902 | |
Na-orotate | 2.50 | g | 2.5 | 14.039 | |
Riboflavin | 15.00 | mg | 0.015 | 0.04 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. |
Last modified: | 08.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
K2HPO4 | 6.95 | 39.902 |
Tryptone | 5 | 70.343 |
Na-orotate | 2.5 | 14.039 |
KH2PO4 | 1.36 | 9.994 |
Na-thioglycolate | 0.5 | 4.382 |
Show more |