DSMZ 249 . FAECALICATENA OROTICA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Faecalicatena orotica DSM 1287

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone
(BD BACTO)
5.00 g 5 70.343
Yeast extract 0.50 g 0.5 -
KH2PO4 1.36 g 1.36 9.994
K2HPO4 6.95 g 6.95 39.902
Na-orotate 2.50 g 2.5 14.039
Riboflavin 15.00 mg 0.015 0.04
Resazurin 1.00 mg 1.0e-3 0.004
Na-thioglycolate 0.50 g 0.5 4.382
Distilled water 1000.00 ml - -
1 Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave.

Metadata

Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Faecalicatena orotica DSM 1287

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 6.95 39.902
Tryptone 5 70.343
Na-orotate 2.5 14.039
KH2PO4 1.36 9.994
Na-thioglycolate 0.5 4.382
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain