249 .
FAECALICATENA OROTICA MEDIUM
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Selected strain: Faecalicatena orotica DSM 1287
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone
(BD BACTO) |
5.00 | g | ||
|
|
Yeast extract | 0.50 | g | ||
|
|
KH2PO4 | 1.36 | g | ||
|
|
K2HPO4 | 6.95 | g | ||
|
|
Na-orotate | 2.50 | g | ||
|
|
Riboflavin | 15.00 | mg | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Na-thioglycolate | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. | |||||