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DSMZ

DSMZ 25 . RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 25 #

ml
Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.25 g 0.25 -
Mannitol 1.50 g 1.5 8.234
Na-gluconate 0.50 g 0.5 2.292
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NH4Cl 0.40 g 0.4 7.478
NaCl 0.40 g 0.4 6.845
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-6 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 4.9, then add:
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 0.02
Biotin
(0.002% in H2O) 		1.00 ml 2.0e-5 8.2e-5
p-Aminobenzoic acid
(0.01% in H2O) 		1.00 ml 1.0e-4 7.3e-4
Sodium thiosulfate pentahydrate
(10% in H2O) 		2.00 ml 0.2 0.806
2 Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 4.9
Equipment needed: Autoclave, Tungsten lamp
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Light

1 strain needs light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 2.292
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain