DSMZ 251 . PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Proteose peptone no. 3 5 g 5 -
Meat extract 3 g 3 -
Glycerol 20 g 20 217.179
Soil extract 150 ml - -
Agar 20 g 20 -
Distilled water 850 ml - -
1 Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation.

Metadata

Last modified: 19.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
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Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycerol 20 217.179
Agar 20 -
Proteose peptone no. 3 5 -
Meat extract 3 -
Soil extract - -
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Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
oxygen aerobe 1 strain
temperature mesophilic 8 strains