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251. PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 -
2 3 g 3 -
3 20 g 20 217.17
4 Soil extract 150 ml - -
5 15 g 15 -
6 850 ml 848.476 47097.5
Instructions:
  1. Adjust pH to 7.0.
Solution occurs in 2 other media (first defined in medium 80 )
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(sieved, air dried)
400 g 416.667 -
3 960 ml 998.207 55408.8
Instructions:
  1. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Garden soil 62.5 -
Glycerol 20 217.17
Agar 15 -
Proteose peptone No. 3 5 -
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen aerobe 1 strain
temperature mesophilic 7 strains

Similar media

80. GLYCEROL-SOIL MEDIUM 94 %
675. BACILLUS THERMANTARCTICUS MEDIUM 85 %

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