DSMZ 252 . STARCH - MINERAL SALT - AGAR (STMS)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Streptomyces gramineus DSM 42058

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble)
10 g 9.99 -
(NH4)2SO4 2 g 1.998 15.12
K2HPO4 1 g 0.999 5.736
MgSO4 x 7 H2O 1 g 0.999 4.053
NaCl 1 g 0.999 17.095
CaCO3 2 g 1.998 19.962
Trace element solution 1 ml - -
Agar 20 g 14.985 44.555
Distilled water 1000 ml - -
1 Adjust pH to 7.2.
Solution occurs in 8 other media (first defined in Medium 84).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Metadata

Last modified: 16.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 500
Strains with modifications: 21
Streptomyces gramineus DSM 42058

No modifications necessary for this strain.

Media similar to medium 252

1240 STARCH-MINERAL SALT-AGAR + 10% NACL
1241 STARCH - MINERAL SALT - AGAR (STMS) + 15% NaCl

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14.985 44.555
Starch 9.99 -
CaCO3 1.998 19.962
(NH4)2SO4 1.998 15.12
NaCl 0.999 17.095
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Cultivation metadata from 500 strains BacDive

pH 6 - 8 42 strains
pH 4 - 6 38 strains
pH 8 - 10 30 strains
pH 10 - 12 17 strains
phyla 2 503 strains
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