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253. MEDIUM FOR ECTOTHIORHODOSPIRA

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.5 3.674
2 0.05 g 0.05 0.34
3 0.80 g 0.8 14.956
4 0.10 g 0.1 0.492
5 180.00 g 180 3079.95
6 20.00 g 20 140.802
7 6.00 g 6 56.61
8 Trace element solution (A)
(A)
1.00 ml - -
9 0.50 g 0.5 -
10 1.00 g 1 6.171
11 800.00 ml 798.566 44327.1
12 Vitamin solution (B) 1.00 ml/l - -
13 Bicarbonate solution (C) 200.00 ml - -
14 Na2S x 9H2O solution (D) 10.00 ml/l - -
Instructions:
  1. Adjust pH to 8.5.
  2. Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min.
  3. After sterilization add 1 ml/l filter-sterilized vitamin solution (B), 200 ml of filter-sterilized solution Bicarbonate solution (C) and 10 ml/l sterilised Na2S x 9H2O solution (D). pH 8.5
Solution occurs in 1 other media (first defined in medium 253 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.8 g 1.8 9.054
2 250.0 mg 0.25 1.051
3 10.0 mg 0.01 0.042
4 10.0 mg 0.01 0.059
5 70.0 mg 0.07 0.354
6 100.0 mg 0.1 0.734
7 500.0 mg 0.5 8.086
8 30.0 mg 0.03 0.124
9 10.0 mg 0.01 0.038
10 1000.0 ml 998.207 55408.8
11 , required for pH adjustment
(1 N)
x - -
Instructions:
  1. For dissolving adjust pH to about 3 with 1 N HCl.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.025 0.102
2 35 mg 0.088 0.716
3 30 mg 0.075 0.201
4 20 mg 0.05 0.365
5 10 mg 0.025 0.123
6 10 mg 0.025 0.052
7 5 mg 0.013 0.008
8 100 ml 249.552 13852.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 14 g 70 833.268
2 200 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 g 100 416.351
2 100 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Light

4 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1009.44 56032.2
NaCl 180 3079.95
Na2SO4 20 140.802
NaHCO3 14 166.654
Na2CO3 6 56.61
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature mesophilic 3 strains
temperature thermophilic 1 strain

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