DSMZ 255 . CLOSTRIDIUM (GS) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Thermoclostridium stercorarium subsp. stercorarium DSM 8532

This strain uses the standard medium without modifications.

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.499 3.667
K2HPO4 x 3 H2O 1.00 g 0.998 5.73
Urea 2.00 g 1.996 33.236
MgCl2 x 6 H2O 0.50 g 0.499 2.454
CaCl2 x 2 H2O 0.05 g 0.05 0.339
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4)
1.25 ml 0.001 0.004
Morpholinopropane sulfonic acid 10.00 g 9.98 47.692
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Yeast extract 6.00 g 5.988 -
L-Cysteine HCl x H2O 1.00 g 0.998 5.682
Cellobiose 5.00 g 4.99 14.578
Distilled water 1000.00 ml - -
1 Dissolve ingredients except cysteine and cellobiose and adjust pH to 7.2. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and readjust pH to 7.2, if necessary. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 13
Strains with modifications: 5
Thermoclostridium stercorarium subsp. stercorarium DSM 8532

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Morpholinopropane sulfonic acid 9.98 47.692
Yeast extract 5.988 -
Cellobiose 4.99 14.578
Urea 1.996 33.236
K2HPO4 x 3 H2O 0.998 5.73
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Cultivation metadata from 13 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 4 strains
pH 4 - 6 2 strains
pH 8 - 10 2 strains
phyla 1 12 strains
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