This strain uses the standard medium without modifications.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 0.50 | g | ||
|
K2HPO4 x 3 H2O | 1.00 | g | ||
|
Urea | 2.00 | g | ||
|
MgCl2 x 6 H2O | 0.50 | g | ||
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
1.25 | ml | ||
|
Morpholinopropane sulfonic acid | 10.00 | g | ||
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
Yeast extract | 6.00 | g | ||
|
L-Cysteine HCl x H2O | 1.00 | g | ||
|
Cellobiose | 5.00 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Dissolve ingredients except cysteine and cellobiose and adjust pH to 7.2. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and readjust pH to 7.2, if necessary. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. |