257
257 .
BDELLOVIBRIO (YP) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Main sol. 257 #
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD Bacto) |
3.0 | g | 3 | - | |
|
Peptone
(BD Bacto) |
0.6 | g | 0.6 | - | |
|
Tris-HCl buffer
(0.05 M, pH 7.5) |
1000.0 | ml | - | 50 | |
|
1
Preparation of double layered agar plates: Bottom layer: Dissolve 12.0 g Bacto agar in 900 ml distilled water and autoclave. After cooling to 60°C add 100 ml of sterile YP medium (autoclaved at 121°C for 15 min) and pour plates. Dry plates at room temperature for 2 - 3 days . Top layer: Grow prey bacterium on agar slants using medium 54. Prepare 5 ml aliquots of 0.6% Bacto agar in distilled water and autoclave in test tubes. After cooling to 45°C in a water bath add 0.5 ml sterile YP medium (autoclaved at 121°C for 15 min) and 0.5 ml cell suspension of host organism, mix and pour top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.
|
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| 2 Inoculate double layer plates the same day when prepared. | |||||
Metadata
| Last modified: | 05.12.22 |
| Source: | DSMZ |
| Synonyms: | BDELLOVIBRIO MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Water bath |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 3 | - |
| Peptone | 0.6 | - |
| Tris-HCl buffer | - | 50 |
