Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(BD Bacto) |
3.0 | g | 3 | - | |
Peptone
(BD Bacto) |
0.6 | g | 0.6 | - | |
Tris-HCl buffer
(0.05 M, pH 7.5) |
1000.0 | ml | - | 50 | |
1
Preparation of double layered agar plates: Bottom layer: Dissolve 12.0 g Bacto agar in 900 ml distilled water and autoclave. After cooling to 60°C add 100 ml of sterile YP medium (autoclaved at 121°C for 15 min) and pour plates. Dry plates at room temperature for 2 - 3 days . Top layer: Grow prey bacterium on agar slants using medium 54. Prepare 5 ml aliquots of 0.6% Bacto agar in distilled water and autoclave in test tubes. After cooling to 45°C in a water bath add 0.5 ml sterile YP medium (autoclaved at 121°C for 15 min) and 0.5 ml cell suspension of host organism, mix and pour top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.
|
|||||
2 Inoculate double layer plates the same day when prepared. |
Last modified: | 05.12.22 |
Source: | DSMZ |
Synonyms: | BDELLOVIBRIO MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Water bath |
Edit in Medium builder |
All strains for this medium: | 4 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Yeast extract | 3 | - |
Peptone | 0.6 | - |
Tris-HCl buffer | - | 50 |