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25. RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

Culture medium recipe

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* Modified for Rhodopila globiformis DSM 161
beta

Anaerobic in light

Equipment needed: Tungsten lamp
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 -
2 1.50 g 1.5 8.234
3 0.50 g 0.5 -
4 0.50 g 0.5 3.674
5 0.40 g 0.4 1.623
6 0.40 g 0.4 7.478
7 0.40 g 0.4 6.844
8 0.05 g 0.05 0.34
9 Trace element solution SL-6 1.00 ml - -
10 1000.00 ml 998.207 55408.8
11
(0.1% in H2O)
5.00 ml - -
12
(0.002% in H2O)
1.00 ml 8.0e-6 3.3e-5
13
(0.01% in H2O)
1.00 ml - -
14 , add 0.2 ml to each 100 ml media
(10 %)
x - -
Instructions:
  1. Adjust pH to 4.9, then add: Fe(III) citrate solution, Biotin solution, and p-Aminobenzoic acid solution (#11-13)
  2. Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 4.9
Equipment needed: Tungsten lamp

Associated strains

All strains for this medium: 1
Strains with modifications: 1
Rhodopila globiformis DSM 161

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 999.205 55464.2
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 -
NH4Cl 0.4 7.478
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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