DSMZ 26 . ACID RHODOSPIRILLACEAE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.20 g 0.2 -
Disodium succinate 1.00 g 1 6.171
Ammonium acetate 0.50 g 0.5 6.487
Fe(III) citrate
(0.1% in H2O)
5.00 ml 0.005 0.02
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NaCl 0.40 g 0.4 6.845
NH4Cl 0.40 g 0.4 7.478
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Vitamin B12
(10 mg in 100 ml H2O)
0.40 ml - -
Trace element solution SL-6 1.00 ml - -
L-Cysteine HCl 0.30 g 0.3 1.903
Resazurin
(0,1%)
0.50 ml 0.005 0.022
Distilled water 1000.00 ml - -
1 Adjust pH to 5.7.
2 Boil the medium for a few minute.
3 Sparge medium with 100% N2 gas mixture to make it anoxic.
4 Fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
5 Autoclave at 121 °C for 20 min.
6 Sterile syringes are used to inoculate and remove samples.
7 Incubate in the light using a tungsten lamp.
Solution occurs in 56 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.7
Equipment needed: Autoclave, Tungsten lamp
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
oxygen anaerobe 7 strains
temperature mesophilic 7 strains