261
261 .
MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 261 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 2 H2O | 4.50 | g | 4.5 | 31.699 | |
| KH2PO4 | 1.50 | g | 1.5 | 11.023 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MnSO4 x H2O | 0.01 | g | 0.01 | 0.059 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Ferric ammonium citrate | 5.00 | mg | 0.005 | - | |
| Trace element solution SL-6 | 3.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C. | |||||
| 2 Incubate at 65C without agitation under 5% (v/v) O2, 10% CO2, 45% H2 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.1 |
| Edit in Medium builder | |
Gas composition
- 45 % H2
- 10 % CO2
- 5 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Na2HPO4 x 2 H2O | 4.5 | 31.699 |
| KH2PO4 | 1.5 | 11.023 |
| NH4Cl | 1 | 18.695 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
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