Culture medium recipe
Vol.
×
Main sol. 262 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 5 | g | 5 | 70.343 | |
| Yeast extract | 15 | g | 15 | - | |
| Glucose | 2 | g | 2 | 11.101 | |
| K2HPO4 | 3 | g | 3 | 17.224 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3 - 7.5. Dissolve glucose in 10 ml of distilled water and sterilize glucose separately. Aseptically add to the basis medium after sterilization. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 - 7.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Yeast extract | 15 | - |
| Tryptone | 5 | 70.343 |
| K2HPO4 | 3 | 17.224 |
| Glucose | 2 | 11.101 |
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