Name: THERMOFILUM PENDENS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 265 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
(NH₄)₂SO₄	1.30	g	1.3	9.838
KH₂PO₄	0.28	g	0.28	2.058
MgSO₄ x 7 H₂O	0.25	g	0.25	1.014
CaCl₂ x 2 H₂O	0.07	g	0.07	0.476
FeCl₃ x 6 H₂O	0.02	g	0.02	0.074
MnCl₂ x 4 H₂O	1.80	mg	0.002	0.009
Na₂B₄O₇ x 10 H₂O	4.50	mg	0.005	-
ZnSO₄ x 7 H₂O	0.22	mg	2.2e-4	7.7e-4
CuCl₂ x 2 H₂O	0.05	mg	5.0e-5	2.9e-4
Na₂MoO₄ x 2 H₂O	0.03	mg	3.0e-5	1.2e-4
VOSO₄ x 2 H₂O	0.03	mg	3.0e-5	1.5e-4
CoSO₄ x 7 H₂O	0.01	mg	1.0e-5	6.5e-5
Yeast extract	2.00	g	2	-
Sucrose	2.00	g	2	5.843
Sulfur (powdered)	10.00	g	10	311.818
Polar lipid fraction , adjust if required (n prepared from Thermoproteus tenax (DSM 2078) or from any other archaebacterium, aqueous suspension)	12.00	ml	-	-
Na₂S x 9 H₂O	0.30	g	0.3	1.249
Distilled water	1000.00	ml	-	-
1 Adjust final pH to 5.2.
2 Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for few minutes, not autoclaved; sucrose (20 ml of 10% w/v solution)-filter-sterilized; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; polar lipid fraction (6 - 12ml)-prepared as described by W. Zillig et al. (1983), Syst. Appl. Microbiol. 4: 79 - 87; Na₂S x 9 H₂O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere.

Compound

Conc. [g/L]

Conc. [mM]

Sulfur

311.818

Sucrose

5.843

Yeast extract

(NH₄)₂SO₄

1.3

9.838

Na₂S x 9 H₂O

0.3

1.249

Last modified:	22.02.22
Source:	DSMZ
Taxonomic range:	Archaea
Medium type:	Complex medium
Final pH:	5.2
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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265 . THERMOFILUM PENDENS MEDIUM