DSMZ 269 . ACIDIPHILIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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* Modified for Acidocella aluminiidurans DSM 108313

Supplement medium with 1.0 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 4.0.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.0 g 2 15.136
KCl 0.1 g 0.1 1.341
K2HPO4 0.5 g 0.5 2.871
MgSO4 x 7 H2O 0.5 g 0.5 2.029
Yeast extract 0.3 g 0.3 -
D-Glucose 1.0 g 1 5.551
Trypticase soy broth* 1.0 g - -
Distilled water 1000.0 ml - -
1 Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 4.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 9
Strains with modifications: 5
Acidocella aluminiidurans DSM 108313

  4.0
  1 g Trypticase soy broth

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 2 15.136
D-Glucose 1 5.551
Trypticase soy broth 1 -
K2HPO4 0.5 2.871
MgSO4 x 7 H2O 0.5 2.029
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Cultivation metadata from 9 strains BacDive

pH 2 - 4 2 strains
pH 4 - 6 2 strains
pH 6 - 8 2 strains
pH 0 - 2 1 strain
phyla 2 9 strains
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