Supplement medium with 1.0 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 4.0.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
(NH4)2SO4 | 2.0 | g | ||
|
KCl | 0.1 | g | ||
|
K2HPO4 | 0.5 | g | ||
|
MgSO4 x 7 H2O | 0.5 | g | ||
|
Yeast extract | 0.3 | g | ||
|
D-Glucose | 1.0 | g | ||
|
Trypticase soy broth | 1.0 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4. |