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26. ACID RHODOSPIRILLACEAE MEDIUM

Culture medium recipe

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* Modified for Rhodoblastus acidophilus DSM 145
beta

Anaerobic in light

Equipment needed: Tungsten lamp Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.2 -
2 1.00 g 1 6.171
3 0.50 g 0.5 6.487
4
(0.1% in H2O)
5.00 ml - -
5 0.50 g 0.5 3.674
6 0.40 g 0.4 1.623
7 0.40 g 0.4 6.844
8 0.40 g 0.4 7.478
9 0.05 g 0.05 0.34
10
(10 mg in 100 ml H2O)
0.40 ml 1.08 0.684
12 Trace element solution SL-6 1.00 ml - -
13 0.30 g 0.3 1.903
14
(0,1%)
0.50 ml - -
15 1000.00 ml 998.207 55408.8
Instructions:
  1. Use medium 27 and reduce the yeast extract concentration to 0.2 g/l. Adjust pH to 5.7. Incubate in the light using a tungsten lamp.
  2. Boil the medium for a few minutes. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121 C for 15 min. Sterile syringes are used to inoculate and remove samples.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.7
Equipment needed: Autoclave, Tungsten lamp

Associated strains

All strains for this medium: 7
Strains with modifications: 7
Rhodoblastus acidophilus DSM 145

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 999.205 55464.2
Vitamin B12 1.08 0.684
Na2-succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
oxygen anaerobe 7 strains
temperature mesophilic 7 strains

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