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27. RHODOSPIRILLACEAE MEDIUM (modified)

Culture medium recipe

Vol.
×
Solution occurs in 2 other media (first defined in medium 27 )
Equipment needed: Tungsten lamp Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 0.298 -
2 1.00 g 0.993 6.128
3 0.50 g 0.497 6.441
4
(0.1% in H2O)
5.00 ml - -
5 0.50 g 0.497 3.649
6 0.40 g 0.397 1.612
7 0.40 g 0.397 6.797
8 0.40 g 0.397 7.426
9 0.05 g 0.05 0.338
10
(10 mg in 100 ml H2O)
0.40 ml 1.072 0.679
11 Trace element solution SL-6 1.00 ml - -
12 0.30 g 0.298 1.89
13
(0,1%)
0.50 ml - -
14 1000.00 ml 991.268 55023.7
Instructions:
  1. Adjust pH to 6.8.
  2. Boil the medium for a few minutes. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples.
  3. Incubate in the light using a tungsten lamp.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Tungsten lamp

Associated strains

All strains for this medium: 80
Strains with modifications: 79

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 992.259 55078.7
Vitamin B12 1.072 0.679
Na2-succinate 0.993 6.128
Ammonium acetate 0.497 6.441
KH2PO4 0.497 3.649
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Cultivation metadata from 80 strains BacDive

halophily not 5 strains
halophily slightly 2 strains
halophily moderate 2 strains
pH 8 - 10 5 strains
pH 6 - 8 5 strains
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